Discover and read the best of Twitter Threads about #Ribosome

Most recents (2)

Developing an #mRNA vaccine is a sure-shot way to success? No! Despite the spectacular performance of #Pfizer & #Moderna vaccines, we also have a #CureVac fiasco. We need to dig in much deeper into the intricacies of developing a highly efficacious mRNA vaccine 1/
There is more than low dose & impact of VOCs behind #CureVac’s failure. And here we once again need to salute the sheer brilliance of @kkariko . Will do a little thread to explain, why! 2/
No doubt, the #CureVac used a much lower #RNA dose. Dose is lower (12 (CureVac) vs 30 (#Pfizer) vs 100 ug (#Moderna)) but no idea how that translates to protein. Need to know how much of a role the lower dose played in the lower efficacy 3/
Read 20 tweets
Super cool combination of modeling, biochemistry, and cell biology to show that co-translational topology of a membrane segment in a viral polyprotein stimulates frame shifting during translation biorxiv.org/content/10.110…. @tfmiller3 #Ribosome #Biochemistry
A weakly hydrophobic transmembrane segment picks one of two orientations - membrane insertion or not. This is stochastic, and in the cases where it passes through the membrane, the forces from the translocon “tugs” on the ribosome to cause a frameshift.
Cool combination of CGMD simulations of protein translocation and some neat labeling / conditional fluorescence experiments to establish distance between TM domain and site of frame shifting needed to stimulate force-dependent frameshift.
Read 3 tweets

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