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Hey #STED, #MINFLUX people,
To continue the public discussion, I list here some of the points I made during my talk.

Summary: MINFLUX is a promising technology but at an early stage of development esp for biological research.

Thread👇

cc @Stefan_W_Hell @BalzarottiFran
Disclaimer 1: I haven't seen or worked with #MINFLUX setup, my arguments mainly on application-side

Disclaimer 2: I asked these questions via email (Sept 2020) and requested raw data and codes but haven't received a reply yet

I hope @rita_strack @naturemethods can help! Image
You can learn more about #MINFLUX from this excellent talk by @Stefan_W_Hell


Also, some papers making an effort in this direction ImageImage
Read 43 tweets
Excited to introduce #3DRCAN for denoising, super resolution and expansion microscopy. Super proud of this colab with Hari Shroff, Jiji Chen et al.

@NIBIBgov @arpcomplex

Preprint: biorxiv.org/content/10.110…

Code: github.com/AiviaCommunity…

#microscopy #aimicroscopy #aivia

1/17
"Three-dimensional residual channel attention networks denoise and sharpen fluorescence microscopy image volumes"

J Chen, H Sasaki, H Lai, Y Su, J Liu, Y Wu, A Zhovmer, C Combs, I Rey-Suarez, H Chang, C Huang, X Li, M Guo, S Nizambad, A Upadhyaya, J Lee, L Lucas, H Shroff.
2/17
We are particularly interested in characterizing the limits of #deeplearning based approaches to image restoration. You will find several experiments comparing several best-in-class approaches: #3DRCAN, #CARE, #SRResNet and #ESRGAN.

#microscopy #aimicroscopy #iSIM

3/17
Read 17 tweets

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